Active Recombinant Mouse Trex1 protein, His&Myc-tagged

• Cat.No.: Trex1-4639M
• Product Overview: Active Recombinant Mouse Trex1 protein(Q91XB0)(1-314aa), fused with N-terminal His tag and C-terminal Myc tag, was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: N-His&C-Myc
• ProteinLength: 1-314aa
• Tag: N-His&C-Myc
• Form: Tris-based buffer, 50% glycerol.
• Bio-activity: TREX1 exonuclease activity liberates free fluorophore from DNA containing a 5'-fluorophore and a 3'-quencher to generate a fluorescent signal. Compound-mediated inhibition of TREX1 activity would be anticipated to reduce fluorescent signal. All reactions were conducted in 20 uL total volumes in 384-well F-bottom polystyrene microplates at 25 °C. Assay buffer consisted of: 25 mM HEPES (pH 7.5), 20 mM KCl, 1 mM DTT, 0.1 mg/mL BSA, 0.01% Triton X-100, 0.25 mM EDTA and 10 mM MgCl2. Reactions were initiated upon the addition dsDNA (oligo 1: /5TEX615/GCTAGGCAG and oligo 2: CTGCCTAGC/3IAbRQSp/ stocks in DNA duplex buffer at a final concentration of 230nM. Fluorescence was measured every two minutes for 60 minutes at 615 nm emission and 570 nm excitation at 25 °C using a NEO plate reader.
• Molecular Mass: 41.1 kDa
• AASequence: MGSQTLPHGHMQTLIFLDLEATGLPSSRPEVTELCLLAVHRRALENTSISQGHPPPVPRPPRVVDKLSLCIAPGKACSPGASEITGLSKAELEVQGRQRFDDNLAILLRAFLQRQPQPCCLVAHNGDRYDFPLLQTELARLSTPSPLDGTFCVDSIAALKALEQASSPSGNGSRKSYSLGSIYTRLYWQAPTDSHTAEGDVLTLLSICQWKPQALLQWVDEHARPFSTVKPMYGTPATTGTTNLRPHAATATTPLATANGSPSNGRSRRPKSPPPEKVPEAPSQEGLLAPLSLLTLLTLAIATLYGLFLASPGQ
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Trex1 three prime repair exonuclease 1 [ Mus musculus ]
• Official Symbol: Trex1
• Synonyms: TREX1; three prime repair exonuclease 1; DNase III; 3-5 exonuclease TREX1; trophoblast expressed 1; three prime exonuclease 1; 1661; AU041952
• Gene ID: 22040
• mRNA Refseq: NM_001012236
• Protein Refseq: NP_001012236