Active Recombinant Mouse Tnfsf13b Protein (Ala127-Leu309), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Tnfsf13b-112M
• Product Overview: Recombinant Mouse Tnfsf13b Protein (Ala127-Leu309) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: BAFF also known as BLYS, TALL-1 and TNFSF13B, which belongs to tumor necrosis factor family. BAFF is a 31.2 kDa type II transmembrane protein containing 285 residues that predominantly produced by myeloid cells, furthermore mouse BAFF shares 72% sequence identity with human BAFF. BAFF has been demonstrated to activate the survival of B cells and the B cell response by binding to BAFFR/BR3. Additionally, BAFF also takes part in regulating B and T cell function via forming two ligands-two receptors pathway through sharing TNFRSF13B/TACI and TNFRSF17/BCMA receptors with APRIL.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce proliferation in mouse B cells. The ED50 for this effect is < 0.5 ng/mL. The specific activity of recombinant mouse BAFF is > 2 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 21.56 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ala127-Leu309
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Tnfsf13b tumor necrosis factor (ligand) superfamily, member 13b [ Mus musculus (house mouse) ]
• Official Symbol: Tnfsf13b
• Synonyms: TNFSF13B; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor ligand superfamily member 13B; B-cell-activating factor; b cell-activating factor; BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; TNFSF20; D8Ertd387e; MGC124060; MGC124061
• Gene ID: 24099
• mRNA Refseq: NM_033622
• Protein Refseq: NP_296371
• UniProt ID: Q9WU72