Active Recombinant Mouse Tnfsf11 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: TNFSF11-860MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant extracellular domain of mouse TNFSF11 (BAA97257.1) (Arg 72-Asp 316) was fused with the Fc region of human IgG1 at the N-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 505
• Form: Lyophilized
• Bio-activity: Immobilized mouse Fc-TNFSF11 at 10 μg/mL (100 μL/well) can bind biotinylated human TNFRSF11B-His, The EC50 of biotinylated human TNFRSF11B-His is 0.07-0.17 μg/mL.
• Molecular Mass: The recombinant mouse TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Glu
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 87 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfsf11 tumor necrosis factor (ligand) superfamily, member 11 [ Mus musculus ]
• Official Symbol: TNFSF11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; TNF-related activation-induc
• Gene ID: 21943
• mRNA Refseq: NM_011613
• Protein Refseq: NP_035743