Active Recombinant Mouse Tnfrsf14 protein(Met1-Gln206), His&hFc-tagged

• Cat.No.: Tnfrsf14-428M
• Product Overview: Recombinant Mouse HVEM (NP_849262.1)(Met 1-Gln 206) was expressed in HEK293, fused with C-terminal His-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Gln206
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse BTLA hFc at 2 μg/ml (100 μl/well) can bind Mouse HVEM Fch, the EC50 of Mouse HVEM Fch is 2-10 ng/mL.
• Molecular Mass: The recombinant mouse HVEM/Fc is a disulfide-linked homodimeric Protein after removal of the signal peptide. The reduced monomer consists of 415 amino acids and predicts a molecular mass of 46.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmHVEM/Fc monomer is approximately 65 kDa due to the glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfrsf14 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) [ Mus musculus ]
• Official Symbol: Tnfrsf14
• Synonyms: TNFRSF14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator); tumor necrosis factor receptor superfamily member 14; herpes virus entry mediator; Atar; HveA; Hvem; Tnfrs14; MGC123498; MGC123499
• Gene ID: 230979
• mRNA Refseq: NM_178931
• Protein Refseq: NP_849262