Active Recombinant Mouse Tgfbr2 Protein (Ile24-Asp184), hFc-tagged

• Cat.No.: Tgfbr2-02M
• Product Overview: Recombinant ouse TGF-beta RII (Ile24 - Asp184) Protein with a Mouse IgG2a (Glu98 - Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Species: Mouse
• Source: NS0
• Tag: mFc
• Protein Length: Ile24-Asp184
• Description: Enables SMAD binding activity; transforming growth factor beta binding activity; and transforming growth factor beta-activated receptor activity. Involved in several processes, including animal organ development; circulatory system development; and positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation. Acts upstream of or within several processes, including animal organ development; cell surface receptor signaling pathway; and positive regulation of tolerance induction. Located in external side of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; embryo mesenchyme; and genitourinary system. Used to study DiGeorge syndrome; Loeys-Dietz syndrome; cleft soft palate; and prostate cancer. Human ortholog(s) of this gene implicated in Loeys-Dietz syndrome 2; Marfan syndrome; gastrointestinal system cancer (multiple); hereditary nonpolyposis colorectal cancer type 6; and pancreatic cancer. Orthologous to human TGFBR2 (transforming growth factor beta receptor 2).
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit TGF-beta 1 activity on HT-2 mouse T cells. The ED50 for this effect is 3-10 ng/mL in the presence of 1 ng/mL of rhTGF-beta 1.
• Molecular Mass: Predicted Molecular Mass: 45.2 kDa (monomer)
  SDS-PAGE: 66 kDa, reducing conditions
• N-terminal Sequence Analysis: Ile24
• Endotoxin: <0.01 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Tgfbr2 transforming growth factor, beta receptor II [ Mus musculus (house mouse) ]
• Official Symbol: Tgfbr2
• Synonyms: Tgfbr2; transforming growth factor, beta receptor II; DNIIR; RIIDN; TBR-II; TbetaRII; TbetaR-II; 1110020H15Rik; TGF-beta receptor type-2; TGF-beta receptor II; TGF-beta receptor type II; TGF-beta type II receptor; TGFR-2; transforming growth factor-beta receptor type II; EC 2.7.11.30
• Gene ID: 21813
• mRNA Refseq: NM_009371
• Protein Refseq: NP_033397
• UniProt ID: Q62312