Active Recombinant Mouse Slit2 Protein, His-tagged

• Cat.No.: Slit2-18M
• Product Overview: Recombinant Mouse Slit2(Thr1114-Ser1521) fused with C-terminal His tag was produced in NS0.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Thr1114
• Form: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit2, immobilized at 625 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
• Molecular Mass: Predicted Molecular Mass: 45 kDa;
  SDS-PAGE: 53-65 kDa, reducing conditions.
• Protein length: Thr1114-Ser1521
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: Slit2 slit homolog 2 (Drosophila) [ Mus musculus ]
• Official Symbol: Slit2
• Synonyms: SLIT2; slit homolog 2 (Drosophila); slit homolog 2 protein; slit-2; neurogenic extracellular slit protein; downregulated during adipocyte differentiation-1; Slil3; Drad-1; mKIAA4141; E030015M03Rik; E130320P19Rik; KIAA4141
• Gene ID: 20563
• mRNA Refseq: NM_178804
• Protein Refseq: NP_848919
• UniProt ID: Q9R1B9