Active Recombinant Mouse SLAM Family Member 7, His-tagged

• Cat.No.: Slamf7-4018M
• Product Overview: A DNA sequence encoding the mouse SLAMF7 ( NP_653122.2 ) extracellular domain ( Met 1 - Gly 224 ) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 1-224 a.a.
• Description: Mouse SLAM family member 7, also known as CRACC, SLAMF7, CD319, CD2-like receptor-activating cytotoxic cells, and CS1, is a single-pass type I membrane protein and a member of the CD2 family of cell surface receptors. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B- cell lines, or T-cell lines. The cytoplasmic domain of SLAMF7 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM)-associated protein (SAP/ SH2D1A). SLAMF7 may play a role in the regulation of B lymphocyte proliferation during immune responses. SLAMF7 is implicated in the activation of NK cell-mediated cytotoxicity. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. In the absence of EAT-2, SLAMF7 potently inhibited natural killer cell function.
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
• Purity: > 95 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to bind biotinylated mouse SLAMF7 in a functional ELISA
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70μ
• Predicted N terminal: Ser 23
• Molecular Mass: The recombinant mouse SLAMF7 consists of 213 amino acids with the predicted molecular mass of 23.7 kDa. As a result of glycosylation, the apparent molecular mass of rm SLAMF7 is approximately 35-50 kDa in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70μ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Slamf7 SLAM family member 7 [ Mus musculus ]
• Official Symbol: Slamf7
• Synonyms: Slamf7; SLAM family member 7; 19A; CS1; 19A24; CRACC; 4930560D03Rik; novel Ly9; leukocyte cell-surface antigen
• Gene ID: 75345
• mRNA Refseq: NM_144539
• Protein Refseq: NP_653122
• UniProt ID: Q8BHK6
• Chromosome Location: 1 E2
• Function: protein binding; receptor activity