Active Recombinant Mouse Serpina1a protein

• Cat.No.: Serpina1a-471M
• Product Overview: Recombinant Mouse Serpina1a protein(XP_003945711.1)(Met1-Lys413) was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• Protein Length: Met1-Lys413
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys (DNP)-NH2.The IC50 value is < 15 nM.
• Molecular Mass: The recombinant mouse Serpina1a comprises 395 amino acids and has a predicted molecular mass of 44.1 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.