Active Recombinant Mouse Ppid Protein, His-tagged

• Cat.No.: Ppid-32M
• Product Overview: Recombinant Mouse Ppid Protein (1-370 aa) is produced by E. coli expression system. This protein is fused with a His-tag.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-370 aa
• Description: Ppid, also known as Peptidyl-prolyl cis-trans isomerase D, is a member of peptidyl-propyl cis-trans isomerase (Pplase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. This protein can bind to the immunosuppressant cyclosporine A and was known that its overexpression the apoptosis in caner cells. Recombinant mouse Ppid protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-Hcl buffer (pH8.0) containing 10% glycerol, 1mM DTT
• Bio-activity: Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
• Molecular Mass: 43.4 kDa (395aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMSHAS PAAKPSNSKN PRVFFDVDIG GERVGRIVLE LFADIVPKTA ENFRALCTGE KGTGSTTGKP LHFKGCPFHR IIKKFMIQGG DFSNQNGTGG
  ESIYGEKFED ENFHYKHDRE GLLSMANAGP NTNGSQFFIT TVPTPHLDGK HVVFGQVIKG LGVARTLENV EVNGEKPAKL CVIAECGELK EGDDWGIFPK DGSGDSHPDF PEDADIDLKD
  VDKILLISED LKNIGNTFFK SQNWEMAIKK YAKVLRYVDS SKAVIEKADR SRLQPIALSC VLNIGACKLK MSNWQGAIDS CLEALEMDPS NTKALYRKAQ GWQGLKEYDQ ALADLKKAQE IAPGDKAIQA ELLKVKQMIK AQKDKEKAVY AKMFA
• Purity: > 90% by SDS - PAGE
• Applications: Enzyme Activity; SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 centigrade to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Ppid peptidylprolyl isomerase D (cyclophilin D) [ Mus musculus ]
• Official Symbol: Ppid
• Synonyms: PPID; PPIase D; rotamase D; cyclophilin 40; Ppif; Ppidl; CYP-40; 4930564J03Rik
• Gene ID: 67738
• mRNA Refseq: NM_026352
• Protein Refseq: NP_080628
• UniProt ID: Q9CR16