Active Recombinant Mouse Pdcd1 Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: Pdcd1-206M
• Product Overview: Biotinylated Mouse PD-1 / PDCD1, Fc Tag is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_032824). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc&Avi
• Predicted N Terminal: Leu 25
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Mouse PD-1, Fc tag with a linear range of 0.15-2.5 ug/mL.
• Molecular Mass: The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 25-167 a.a.

Gene Information

• Gene Name: Pdcd1 programmed cell death 1 [ Mus musculus ]
• Official Symbol: Pdcd1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101
• Gene ID: 18566
• mRNA Refseq: NM_008798
• Protein Refseq: NP_032824