Active Recombinant Mouse NRP1 protein(Phe22-Pro856), His-tagged

• Cat.No.: NRP1-0565M
• Product Overview: Recombinant Mouse NRP1 (NP_032763.2) (Met1-Pro856) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Phe22-Pro856
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized VEGF165 at 10 μg/mL (100 μL/well) can bind mNRP1h, the EC50 of mNRP1h is 30-120 ng/mL.
• Molecular Mass: The recombinant mouse NRP1 consists of 846 amino acids and predicts a molecular mass of 95.12 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nrp1 neuropilin 1 [ Mus musculus ]
• Official Symbol: NRP1
• Synonyms: NRP1; neuropilin 1; neuropilin-1; A5 protein; transmembrane receptor; Neuropilin-1 precursor (A5 protein); Nrp; NP-1; Npn1; NPN-1; C530029I03
• Gene ID: 18186
• mRNA Refseq: NM_008737
• Protein Refseq: NP_032763