Active Recombinant Mouse Lifr Protein, His-tagged

• Cat.No.: Lifr-021M
• Product Overview: Recombinant mouse LIFR alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect cells
• Tag: His
• Description: Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
• Form: Liquid
• Bio-activity: Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤ 1μg/mL with Mouse LIF.
• Molecular Mass: 89.6 kDa
• AA Sequence: LKRGVQDLKCTTNNMRVWDCTWPAPLGVSPGTVKDICIKDRFHSCHPLETTNVKIPALSPGDHEVTINYLNGFQSKFTLNEKDVSLIPETPEILDLSADFFTSSLLLKWNDRGSALPHPSNATWEIKVLQNPRTEPVALVLLNTMLSGKDTVQHWNWTSDLPLQCATHSVSIRWHIDSPHFSGYKEWSDWSPLKNISWIRNTETNVFPQDKVVLAGSNMTICCMSPTKVLSGQIGNTLRPLIHLYGQTVAIHILNIPVSENSGTNIIFITDDDVYGTVVFAGYPPDVPQKLSCETHDLKEIICSWNPGRITGLVGPRNTEYTLFESISGKSAVFHRIEGLTNETYRLGVQMHPGQEIHNFTLTGRNPLGQAQSAVVINVTERVAPHDPTSLKVKDINSTVVTFSWYLPGNFTKINLLCQIEICKANSKKEVRNATIRGAEDSTYHVAVDKLNPYTAYTFRVRCSSKTFWKWSRWSDEKRHLTTEATPSKGPDTWREWSSDGKNLIVYWKPLPINEANGKILSYNVSCSLNEETQSVLEIFDPQHRAEIQLSKNDYIISVVARNSAGSSPPSKIASMEIPNDDITVEQAVGLGNRIFLTWRHDPNMTCDYVIKWCNSSRSEPCLLDWRKVPSNSTETVIESDQFQPGVRYNFYLYGCTNQGYQLLRSIIGYVEELAPIVAPNFTVEDTSADSILVKWDDIPVEELRGFLRGYLFYFQKGERDTPKTRSLEPHHSDIKLKNITDISQKTLRIADLQGKTSYHLVLRAYTHGGLGPEKSMFVVTKENS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -100 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Lifr LIF receptor alpha [ Mus musculus (house mouse) ]
• Official Symbol: Lifr
• Synonyms: Lifr; LIF receptor alpha; LIF; AW061234; A230075M04Rik; leukemia inhibitory factor receptor; D-factor/LIF receptor; LIF-R; leukemia inhibitory factor receptor alpha chain; soluble differentiation-stimulating factor receptor
• Gene ID: 16880
• mRNA Refseq: NM_013584
• Protein Refseq: NP_038612
• UniProt ID: P42703