Active Recombinant Mouse LAG3 protein(Met1-Arg432), hFc-tagged

• Cat.No.: LAG3-673M
• Product Overview: Recombinant Mouse Lag3 (NP_032505.1) (Met1-Arg432) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg432
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Mouse LAG3 Protein(hFc Tag) at 2 μg/mL (100 μL/well) can bind Mouse FGL1 (His Tag), the EC50 is 180-700 ng/mL.
• Molecular Mass: The recombinant mouse Lag3 consists of 648 amino acids and predicts a molecular mass of 71.1 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lag3 lymphocyte-activation gene 3 [ Mus musculus ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; Ly66; CD223; LAG-3
• Gene ID: 16768
• mRNA Refseq: NM_008479
• Protein Refseq: NP_032505