Active Recombinant Mouse L1cam Protein, Fc-tagged

• Cat.No.: L1cam-01M
• Product Overview: Recombinant mouse L1CAM (Met1-Glu1123) with a Phe176Leu Substitution fused with a Human IgG1 (Pro100-Lys330) at C-terminus was expressed in Mouse myeloma cell line NS0.

Specification

• Description: Biased expression in CNS E18 (RPKM 43.2), whole brain E14.5 (RPKM 35.1) and 12 other tissues.
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. Recombinant Mouse L1CAM Fc Chimera immobilized at 2.5 μg/mL with 100 μL/well will induce >40% cell adhesion.
  Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: 150.5 kDa
• N-terminal Sequence Analysis: Ile20
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 1-1123 a.a.

Gene Information

• Gene Name: L1cam L1 cell adhesion molecule [ Mus musculus (house mouse) ]
• Official Symbol: L1cam
• Synonyms: L1cam; L1 cell adhesion molecule; L; L1; L1-N; NCAM; CD171; NCAM-L1; N-CAM-L1; neural cell adhesion molecule L1
• Gene ID: 16728
• mRNA Refseq: NM_008478
• Protein Refseq: NP_032504
• UniProt ID: P11627