Active Recombinant Mouse Interleukin 4

• Cat.No.: Il4-469M
• Product Overview: Recombinant IL-4 is produced in E.coli as a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13,500 Daltons.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Mouse Interleukin-4, also known as BCDF or BCGF, is a pleiotropic immunoregulatory cytokine. IL-4 activity is mediated through the IL-4R receptor.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Recombinant mouse IL-4 is fully biologically active when compared to standards. The ED50, as determined by dose-dependent induction of HT-2 cell proliferation, is 2.5 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Pathways: Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; T cell receptor signaling pathway

Gene Information

• Gene Name: Il4 interleukin 4 [ Mus musculus ]
• Official Symbol: Il4
• Synonyms: Il4; interleukin 4; Il-4; BSF-1; interleukin-4; OTTMUSP00000005817; OTTMUSP00000005817; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; lymphocyte stimulatory factor; IL-4; BCGF-1; binetrakin; BCGF1; BSF1; pitrakinra; Binetrakin; MGC79402; B_cell stimulatory factor 1; Pitrakinra
• Gene ID: 16189
• mRNA Refseq: NM_021283
• Protein Refseq: NP_067258
• UniProt ID: P07750
• Chromosome Location: 11 B1.3; 11 29.0 cM
• Function: interleukin-4 receptor binding; protein binding