Active Recombinant Mouse Interleukin 12b, His-tagged

• Cat.No.: Il12b-5647M
• Product Overview: RecombinantMouse Il12b protein was expressed in Murine myeloma cellline. Gln20-Glu561,with a C-terminal 6-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 20-561 a.a.
• Description: Il12b isa 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF Rfamily ofcytokine receptors. Il12b is a common subunit of both the IL-12 andIL-23 receptor complexes which play distinct but related roles in T cell mediatedinflammatory reactions. Mature mouse Il12b contains a 546 amino acid (aa) extracellulardomain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmicdomain. Within the ECD, mouse Il12b shares 85% and 52% aa sequence identitywith rat and human Il12b, respectively. It shares 16%-21% aa sequenceidentity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 andIL-23 are disulfide linked heterodimeric cytokines that share a common p40subunit.
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Gln20 predicted
• MolecularWeight: 61.3 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rmIL-12 R 1 at 10 μg/mL(100 μL/well) can bind rmIL-12 with a linear range of 0.3-20 μg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 95-110kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il12b interleukin 12b [ Musmusculus ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin12b; p40;Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40;IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor40 kDa subunit
• Gene ID: 16160
• mRNA Refseq: NM_008352
• Protein Refseq: NP_032378
• UniProt ID: Q60837
• Chromosome Location: 11
• Pathway: Africantrypanosomiasis; Allograft rejection; Amoebiasis; Chagas disease (Americantrypanosomiasis); Cytokine-cytokine receptor interaction; Herpes simplexinfection; Influenza A; Jak-STAT signaling pathway; Legionellosis; Leishmaniasis;Measles; Pertussis; RIG-I-like receptor signaling pathway; Toll-like receptorsignaling pathway; Toxoplasmosis
• Function: contributes_tocytokine activity; cytokine receptor activity; contributes_to cytokinereceptor binding; identical protein binding; interleukin-12 alpha subunitbinding; protein binding; protein heterodimerization activity