Active Recombinant Mouse Intercellular Adhesion Molecule 1, Fc-tagged

• Cat.No.: Icam1-5640M
• Product Overview: RecombinantMouse Icam1 protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Mouse Icam1 Gln28-Asn485(N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 28-485 a.a.
• Description: Icam1binds the leukocyte integrins LFA-1 and Mac-1. Icam1 expression is weak onleukocytes, epithelial and resting endothelial cells, as well as some othercell types, but expression can be stimulated by IFN-γ, TNF-α, IL-1β and LPS.Mouse and human Icam1 share approximately 54% amino acid identity.
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS.
• N-terminalSequence: Gln28
• MolecularWeight: 76.8 kDa (monomer)
• Activity: Measuredby the ability of the immobilized protein to support the adhesion ofPMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemiacells. When 5x104cells/well are added to rmICAM/Fc Chimera coatedplates (12.5 μg/Ml with 100 μL/well), approximately 65-95% will adhere after1 hour incubation at 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 110-130kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 0.4 mg/mLin sterile PBS
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from dateof receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Icam1 intercellular adhesionmolecule 1 [ Mus musculus ]
• Official Symbol: Icam1
• Synonyms: Icam1; intercellularadhesion molecule 1; CD54; Ly-47; Icam-1; MALA-2; myD10; Intercellular adhesion molecule
• Gene ID: 15894
• mRNA Refseq: NM_010493
• Protein Refseq: NP_034623
• UniProt ID: Q3U8M7
• Chromosome Location: 9
• Pathway: Adaptive ImmuneSystem; African trypanosomiasis; Cell adhesion molecules (CAMs); HTLV-Iinfection; IL-2 Signaling Pathway; IL-5 Signaling Pathway; Immune System; InfluenzaA; Integrin cell surface interactions; Leukocyte transendothelial migration; Malaria;Natural killer cell mediated cytotoxicity; PluriNetWork; Rheumatoid arthritis;Signal Transduction
• Function: cell surface binding;integrin binding; protein complex binding