Active Recombinant Mouse IL4 protein, His-tagged
Cat.No. : | IL4-353M |
Product Overview : | Recombinant Mouse IL4 protein(P07750)(Met 1-Ser140, Q136D, Y139D), fused with His tag, was expressed in Insect Cells. |
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Species : | Mouse |
Source : | Insect Cells |
Tag : | His |
Protein Length : | Met 1-Ser140, Q136D, Y139D |
Form : | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc, The EC50 of rat IL4R-Fc is 0.12-0.29 μg/mL.2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4. |
Molecular Mass : | The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | Il4 interleukin 4 [ Mus musculus ] |
Official Symbol | IL4 |
Synonyms | IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1; |
Gene ID | 16189 |
mRNA Refseq | NM_021283 |
Protein Refseq | NP_067258 |
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Not For Human Consumption!
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