Active Recombinant Mouse Il3ra Protein, Fc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Il3ra-5661MAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant mouse Il3ra protein was expressed in Spodopterafrugiperdawith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl3ra Ser17-Lys331 (N-terminus).

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: Fc
• Protein Length: 17-331 a.a.
• Description: Il3ra isa pleiotropic cytokine produced primarily by activated T cells or mast cells.Il3ra stimulates the proliferation and differentiation of hemopoietic cellsincluding the pluripotent hematopoietic stem cells as well as various lineage-committedcells. The biological effects of Il3ra on the various cell types are mediatedby the binding of Il3ra to specific cell surface receptor complexes. Thefunctional high-affinity IL-3 receptor is a heterodimer consisting of aligand binding α subunit and the β subunit. The α subunit alone binds Il3rawith low affinity.
• Form: Lyophilized
• Bio-activity: Measuredby its ability to inhibit the IL-3-induced proliferation of NFS-60 mouse myeloidcells. The ED50 for this effect is typically 0.03-0.15 μg/mL in the presenceof 2.5 μg/mL Recombinant Mouse IL-3 Rβ Fc Chimera and 2 ng/mL of RecombinantMouse Il3ra.
• Molecular Mass: 61.0 kDa (monomer); SDS-PAGE: 70-80kDa, reducing conditions.
• N-terminal Sequence Analysis: Ser 17
• Endotoxin: < 1.0 EU/ μg of the protein by the LAL method.
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• Reconstitution: 100 μg/mL in sterile PBS

Gene Information

• Gene Name: Il3ra interleukin 3 receptor,alpha chain [ Mus musculus ]
• Official Symbol: Il3ra
• Synonyms: Il3ra; interleukin 3receptor, alpha chain; CD123; SUT-1; CDw123; interleukin-3 receptor subunit alpha; IL-3RA; IL-3R-alpha; Il-3 alpha subunit; IL-3R subunit alpha; IL-3 receptor alpha chain; IL-3 receptor subunit alpha; IL-3 receptor alpha SP2 isoform; interleukin-3 receptor class II alpha chain
• Gene ID: 16188
• mRNA Refseq: NM_008369
• Protein Refseq: NP_032395
• UniProt ID: P26952