Active Recombinant Mouse Il12b Protein

• Cat.No.: Il12b-1235M
• Product Overview: Purified recombinant protein of Mouse interleukin 12b (Il12b) (Met23-Ser335) without tag was was expressed Sf9 cell.

Specification

• Species: Mouse
• Source: Insect Cells
• Protein Length: 23-335 a.a.
• Description: This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23.
• Bio-activity: The ED50 is 1- 4 ng/ml corresponding to a specific activity of 1.0 - 0.25 x 10^6 units/mg, determined by the dose dependent inhibition of IL-12-dependent IFNγ production.
• Molecular Mass: 35.8 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 µg/mL in specified buffer
• Storage Buffer: 20 mM Tris-HCl, pH8.0, 0.1 M NaCl

Gene Information

• Gene Name: Il12b interleukin 12b [ Mus musculus (house mouse) ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin 12b; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40 subunit; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin-12 p40 subunit
• Gene ID: 16160
• mRNA Refseq: NM_001303244
• Protein Refseq: NP_001290173
• UniProt ID: P43432