Active Recombinant Mouse Il12a Protein, His-Tagged

Description :	Active IL-12 is a p70 disulfide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
Source :	Escherichia coli
Species :	Mouse
Tag :	His
Form :	Lyophilized powder
AA Sequence :	MRVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLM MTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINR VMGYLSSA with polyhistidine tag at the C-terminus
Endotoxin :	<0.1 EU per 1 μg of the protein by the LAL method.
Bio-activity :	Measure by its ability to induce proliferation in T-cell enriched PBMC. The ED50 for this effect is <0.2 ng/mL.
Purity :	>98% as determined by SDS-PAGE. Ni-NTA chromatography
Storage Buffer :	The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Reconstitution :	It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Storage :	Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes :	Please use within one month after protein reconstitution.

Gene Name	Il12a interleukin 12a [ Mus musculus (house mouse) ]
Official Symbol	Il12a
Synonyms	p35; Ll12a; Il-12a; IL-12p35
Gene ID	16159
mRNA Refseq	NM_001159424.3
Protein Refseq	NP_001152896.2
UniProt ID	P43431