Active Recombinant Mouse Gpc3, His-tagged

• Cat.No.: Gpc3-5627M
• Product Overview: Recombinant Mouse Gpc3(Gln25-Met557 & Ser358-Met557), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.

Specification

• Description: Glypican-3 is a protein that in humans is encoded by the GPC3 gene. The protein encoded by this gene is a member of the glypican family.
• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gln25 predicted (No result obtained, sequencing might be blocked) & Ser358
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Glypican 3 is coated at 5 µg/mL (100 µL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is found to be approximately 0.75 - 3.75 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 61.3 kDaSDS-PAGE: 66-98 kDa & 30-40 kDa, reducing conditions
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 ºC as supplied.1 month, 2 to 8 ºC under sterile conditions after reconstitution.3 months, -20 to -70 ºC under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 µg/mL in PBS.
• Protein length: 25-557;358-557 a.a.

Gene Information

• Gene Name: Gpc3 glypican 3 [ Mus musculus (house mouse) ]
• Official Symbol: Gpc3
• Synonyms: Gpc3; glypican 3; OCI-5; glypican-3
• Gene ID: 14734
• mRNA Refseq: NM_016697
• Protein Refseq: NP_057906
• UniProt ID: Q8CFZ4
• Chromosome Location: X; X A3.3
• Pathway: A tetrasaccharide linker sequence is required for GAG synthesis; Defective B4GALT7 causes EDS; Defective CHST3 causes SEDCJD
• Function: heparan sulfate proteoglycan binding; peptidase inhibitor activity; peptidyl-dipeptidase inhibitor activity; protein binding