Active Recombinant Mouse Glypican 5, His-tagged

• Cat.No.: Gpc5-5628M
• Product Overview: RecombinantMouse Gpc5 protein was expressed in Murine myeloma cell line. Gly26-Gly549,with a C-terminal 6-His tag.

Specification

• Cat. No.: Gpc5-5628M
• Description: Theglypicans (glypiated proteoglycans) are a small multigene family ofGPI-linked proteoglycans that likely play a key role in embryonicmorphogenesis. There are six known mammalian glypicans. They all share a common-sizedprotein core of 60-70 kDa, an N-terminus which likely forms a compact globulardomain, 14 conserved cysteines that form multiple intrachain disulfide bonds,and a number of C-terminal N-and O-linked carbohydrate attachment sites.Based on exon organization and the location of O-linked glycosylation sites,at least two subfamilies of glypicans are known, with one subfamily containingglypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Gly26
• MolecularWeight: 59.1 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc5 at5μg/mL (100μL/well) can bind rhFGF-basic with a linear range of 0.16-10ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 61-65kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
• Protein length: 26-549 a.a.

Gene Information

• Gene Name: Gpc5 glypican 5 [ Musmusculus ]
• Official Symbol: Gpc5
• Synonyms: Gpc5; glypican 5; A230034F01Rik;glypican-5
• Gene ID: 103978
• mRNA Refseq: NM_175500
• Protein Refseq: NP_780709
• UniProt ID: O70206
• Chromosome Location: 14
• Function: heparan sulfateproteoglycan binding