Active Recombinant Mouse Fibroblast Growth Factor Receptor 3, Fc-tagged

• Cat.No.: Fgfr3-536M
• Product Overview: Recombinant Mouse Fgfr3protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Ala33-Tyr367 (N-terminus).

Specification

• Cat. No.: Fgfr3-536M
• Description: Fibroblast growthfactors (FGFs) comprise a family of at least eighteen structurally relatedproteins that are involved in a multitude of physiological and pathologicalcellular processes, including cell growth, differentiation, angiogenesis,wound healing and tumorgenesis. The biological activities of the FGFs aremediated by a family of type I transmembrane tyrosine kinases which undergodimerization and autophosphorylation after ligand binding. Four distinctgenes encoding closely related FGF receptors, FGF R1-4, are known.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ala33
• Molecular Weight: 63 kDa
• Activity: Measuredby its ability to inhibit FGF acidicdependent proliferation of NR6R-3T3 mousefibroblasts. The ED50 for this effect is typically 2-6 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 100-110kDa, reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 33-367 a.a.

Gene Information

• Gene Name: Fgfr3 fibroblast growth factorreceptor 3 [ Mus musculus ]
• Official Symbol: Fgfr3
• Synonyms: Fgfr3;fibroblast growth factor receptor 3; FR3; Mfr3; sam3; CD333; Flg-2; HBGFR;Fgfr-3; fibroblast growth factor receptor 3; heparin-binding growth factorreceptor; EC 2.7.10.1; mCG_16331
• Gene ID: 14184
• mRNA Refseq: NM_001163215
• Protein Refseq: NP_001156687
• UniProt ID: Q61851
• Chromosome Location: 5 B; 5 20.0 cM
• Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; IRS-mediated signalling; IRS-related events; Insulin receptorsignalling cascade; MAPK signaling pathway; PI-3K cascade; Pathways incancer; Phospholipase C-mediated cascade; Regulation of Actin Cytoskeleton; Signalingby FGFR; Signaling by Insulin receptor; Bladder cancer; EndochondralOssification
• Function: ATP binding;fibroblast growth factor binding; fibroblast growth factor-activated receptoractivity; kinase activity; nucleotide binding; protein binding; proteintyrosine kinase activity; receptor activity; transferase activity;transmembrane receptor protein tyrosine kinase activity