Active Recombinant Mouse Fibroblast Growth Factor 1

• Cat.No.: Fgf1-565M
• Product Overview: M-FGF-acidic is non-glycosylated heparin binding protein comprised of 141 amino acid residues with a molecular weight of 15.9 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Recombinant Mouse Fibroblast Growth Factor-acidic is one of over 20 members of the FGF family. The fibroblast growth factors play a central role in prenatal development and growth and regeneration of cells during the proliferation and differentiation stages.
• Form: Lyophilized at a concentration of 1 mg/ml in 5 mM NaP, 7.5 + 50 mM NaCl.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Recombinant Human FGF-alpha is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.04ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDTEGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Fgf1 fibroblast growth factor 1 [ Mus musculus ]
• Official Symbol: Fgf1
• Synonyms: Dffrx; Fam; Fgf-1; Fgfa; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a; Heparin-binding growth factor 1; Acidic fibroblast growth factor; aFGF; ECGFB; FGF-alpha; FGFA; HBGF1; AFGF; Beta-endothelial cell growth factor; Heparin binding growth factor 1 precursor; endothelial cell growth factor alpha; endothelial cell growth factor beta; fibroblast growth factor 1 (acidic); heparin binding growth factor 1; Fgf1; fibroblast growth factor 1Dffrx; Fam; Fgf-1; Fgfa; HBGF-1; ECGF-beta; FIBP; FGFIBP; FIBP-1; ECGF; ECGFA; GLIO703; FGF1; FGF-a; Heparin-binding growth factor 1; Acidic fibroblast growth factor; aFGF; ECGFB; FGF-alpha; FGFA; HBGF1; AFGF; Beta-endothelial cell growth factor; Heparin binding growth factor 1 precursor; endothelial cell growth factor alpha; endothelial cell growth factor beta; fibroblast growth factor 1 (acidic); heparin binding growth factor 1
• Gene ID: 14164
• mRNA Refseq: NM_010197
• Protein Refseq: NP_034327
• UniProt ID: P61148
• Chromosome Location: 18 19.0 cM
• Pathway: MAPK signaling pathway; Melanoma; Regulation of actin cytoskeleton
• Function: growth factor activity; heparin binding