Active Recombinant Mouse Fgl2 Protein, His-tagged

• Cat.No.: Fgl2-001M
• Product Overview: Recombinant Mouse Fgl2 Protein (Val20-Pro432 & Asp31-Pro432) both with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: 20-432;31-432 a.a.
• Description: Converts prothrombin to thrombin.
• Form: Disulfide-linked homotetramer
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of mouse splenic T lymphocytes. The soluble Recombinant Mouse FGL2 inhibited the proliferation with a typical ED50 value of 1-4 μg/mL.
• Molecular Mass: 47.7 kDa (monomer)
• N-terminal Sequence Analysis: Val20 & Asp31
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: Fgl2 fibrinogen-like protein 2 [ Mus musculus (house mouse) ]
• Official Symbol: Fgl2
• Synonyms: Fgl2; fibrinogen-like protein 2; AI385601; musfiblp; fibroleukin; associated with MHV-3 induced prothrombinase activity; cytotoxic T-lymphocyte-specific protein; prothrombinase
• Gene ID: 14190
• mRNA Refseq: NM_008013
• Protein Refseq: NP_032039
• UniProt ID: P12804