Active Recombinant Mouse Fcgr2b protein(Met1-Arg217), His&Avi-tagged, Biotinylated

• Cat.No.: Fcgr2b-2968M
• Product Overview: Biotinylated Recombinant Mouse FGGR2B (NP_001070657.1)(Met 1-Arg 217) was expressed in HEK293, fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&Avi
• Protein length: Met1-Arg217
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its ability to bind human IgG1 in a functional ELISA. 2. Loaded Biotinylated Mouse FcγRIIB / CD32b recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.04 μM as determined in a BLI assay (ForteBio Octet Red384).
• Molecular Mass: The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr2b Fc receptor, IgG, low affinity IIb [ Mus musculus ]
• Official Symbol: Fcgr2b
• Synonyms: FCGR2B; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma Fc region receptor II-b; fcRII; FcgammaRIIB; Fc-gamma-RII; fc-gamma RII; Fc gamma RIIB; fc-gamma-RIIB; fc gamma receptor IIB; lymphocyte antigen 17; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
• Gene ID: 14130
• mRNA Refseq: NM_001077189
• Protein Refseq: NP_001070657