Active Recombinant Mouse Erbb4 Protein, His tagged

• Cat.No.: Erbb4-7416M
• Product Overview: Recombinant Mouse Erbb4 protein (Q61527) (Met1-Leu652) is produced by HEK293 Cells expression system. This protein is fused with a His tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu652
• Form: Lyophilized from a 0.2μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse ERBB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.55-1.29 μg/ml.
• Molecular Mass: The recombinant mouse ERBB4 comprises 638 amino acids and has a predicted molecular mass of 71.5 kDa. The apparent molecular mass of the protein is approximately 113 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Erbb4 v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) [ Mus musculus ]
• Official Symbol: Erbb4
• Synonyms: ERBB4; v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian); Her4; c-erbB-4
• Gene ID: 13869
• mRNA Refseq: NM_010154
• Protein Refseq: NP_034284
• UniProt ID: Q61527