Active Recombinant Mouse Epidermal Growth Factor

• Cat.No.: Egf-557M
• Product Overview: Epidermal Growth Factor Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Recombinant Mouse Epidermal Growth Factor has an effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE. Analytical HPLC.
• Bio-activity: Mouse EGF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is 0.02 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE. Analytical HPLC
• Protein Content determined by: UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus ]
• Official Symbol: Egf
• Synonyms: AI790464; URG; urogastrone; Pro-epidermal growth factor precursor; epidermal growth factor; epidermal growth factor (beta-urogastrone); Egf
• Gene ID: 13645
• mRNA Refseq: NM_010113
• Protein Refseq: NP_034243
• UniProt ID: P01132
• Chromosome Location: 3 G3; 3 65.2 cM
• Pathway: Cytokine-cytokine receptor interaction; Endometrial cancer; ErbB signaling pathway; Focal adhesion; Gap junction; Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Prostate cancer; Regulation of actin cytoskeleton
• Function: calcium ion binding; growth factor activity