Active Recombinant Mouse EphB2 protein, His-tagged

• Cat.No.: Ephb2-497M
• Product Overview: Recombinant Mouse EphB2 protein(NP_034272.1)(Met1-Lys540), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EPHB2-His at 10 μg/mL (100 μL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.01-0.03 μg/mL.
• Molecular Mass: The recombinant mouse EPHB2 comprises 533 amino acids and has a predicted molecular mass of 59.3 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Lys540
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ephb2 Eph receptor B2 [ Mus musculus ]
• Official Symbol: EphB2
• Synonyms: EPHB2; Eph receptor B2; ephrin type-B receptor 2; neural kinase; nuk receptor tyrosine kinase; tyrosine-protein kinase receptor EPH-3; tyrosine-protein kinase receptor SEK-3; Drt; Erk; Nuk; Cek5; Hek5; Qek5; Sek3; ETECK; Prkm5; Tyro5
• Gene ID: 13844
• mRNA Refseq: NM_010142
• Protein Refseq: NP_034272