Active Recombinant Mouse Eph Receptor B6, His-tagged

• Cat.No.: Ephb6-459M
• Product Overview: Recombinant Mouse Ephb6protein was expressed in Murine myeloma cell line using a C-terminal His tag.Leu33-Ser587.

Specification

• Cat. No.: Ephb6-459M
• Description: Ephb6, also known asMep, is a member of the Eph receptor family which binds members of the ephrinligand family. There are two classes of receptors, designated A and B. Both theA and B class receptors have an extracellular region consisting of a globulardomain, a cysteinerich domain, and two fibronectin type III domains. This is followedby the transmembrane region and cytoplasmic region.
• Source: Mammalian cells
• Form: Lyophilized from a 0.2μm filtered solution in Tris and NaCl.
• N-terminalSequence: Leu33
• Molecular Weight: 87.3 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rmEphb6/Fc Chimera at2 μg/mL (100 μL/well) can bind rmEphrinB2/Fc Chimera with a linear range of 0.078-5ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution.
• Protein length: 33-587 a.a.

Gene Information

• Gene Name: Ephb6 Eph receptorB6 [ Mus musculus ]
• Official Symbol: Ephb6
• Synonyms: Ephb6; Ephreceptor B6; Mep; Cekl; ephrin type-B receptor 6; ephrin type A receptor; chickeneph/elk receptor-like protein; tyrosine-protein kinase-defective receptor EPH-6;EC 2.7.10.1
• Gene ID: 13848
• mRNA Refseq: NM_001146351
• Protein Refseq: NP_001139823
• UniProt ID: O08644
• Chromosome Location: 6 B2.1; 6
• Pathway: Axon guidance
• Function: ATP binding; ephrin receptoractivity; nucleotide binding; protein kinase activity; receptor activity; transferaseactivity