Active Recombinant Mouse EGF protein, Fc-tagged

• Cat.No.: EGF-1410M
• Product Overview: Recombinant Mouse EGF protein(Asn977-Arg1029), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5.
• Bio-activity: Immobilized Mouse EGF R, His Tag at 5 μg/mL (100 μL/well) can bind Mouse EGF, Fc Tag with a linear range of 0.2-5 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 32.5 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asn977-Arg1029
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 977-1029 a.a.

Gene Information

• Gene Name: Egf epidermal growth factor [ Mus musculus ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); AI790464
• Gene ID: 13645
• mRNA Refseq: NM_010113
• Protein Refseq: NP_034243