Active Recombinant Mouse Cysteine-Rich Secretory Protein 3, His-tagged

• Cat.No.: Crisp3-5611M
• Product Overview: Recombinant Mouse Crisp3protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Gln20-Cys241.

Specification

• Cat. No.: Crisp3-5611M
• Description: Crisp3 is one ofthree CRISPs found in mammalian exocrine secretions and granulocytes that mayplay a role in innate immunity. CRISPs and several snake, insect, and lizardvenom proteins are characterized by 16 invariant cysteine residues.Structurally, they consist of an N-terminal SCP domain, a hinge region, and acysteine-rich domain. Human Crisp3 is produced by salivary, pancreas,prostate, and lacrimal glands, as well as spermatozoa and mature spermatids.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln20 predicted(Nsequencing might be blocked)
• Molecular Weight: 26 kDa
• Activity: Measuredby its ability to inhibit proliferation/survival of 3A-sub E human placentacells. The ED50 for this effect is typically 1.3-5.2 μg/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 30-40kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 250 μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
• Protein length: 20-241 a.a.

Gene Information

• Gene Name: Crisp3 cysteine-rich secretoryprotein 3 [ Mus musculus ]
• Official Symbol: Crisp3
• Synonyms: Crisp3; cysteine-richsecretory protein 3; Aeg2; CRS3; SGP28; CRISP-3; SCP 2; Aeg-2; sperm-coatingglycoprotein 2; acidic epididymal glycoprotein 2
• Gene ID: 11572
• mRNA Refseq: NM_009639
• Protein Refseq: NP_033769
• UniProt ID: Q03402
• Chromosome Location: 17 B2; 17 21.0 cM