Active Recombinant Mouse Cystatin B, His-tagged

• Cat.No.: Cstb-4002M
• Product Overview: A DNA sequence encoding the mouse CTSB ( P10605 ) ( Met 1 - Phe 339 ) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Mouse
• Tag: His
• ProteinLength: 1-339 a.a.
• Description: Mouse cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures.
• Form: Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.
• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC ( R&D Systems, Catalog # ES008 ). The specific activity is > 1,000 pmoles / min / µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70μ
• Predicted N terminal: His 18
• Molecular Mass: The secreted recombinant mouse CTSB ( pro form ) consists of 333 amino acids and has a predicted molecular mass of 36.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTSB is approximately 35-43 kDa due to glycosylation
• Storage: Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Cstb cystatin B [ Mus musculus ]
• Official Symbol: Cstb
• Synonyms: Cstb; Stfb; AA960480; cystatin B; cystatin-B; stefin-B
• Gene ID: 13014
• mRNA Refseq: NM_007793
• Protein Refseq: NP_031819
• UniProt ID: Q62426
• Chromosome Location: 10 C1; 10 42.0 cM
• Function: cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding