Active Recombinant Mouse Cystatin B, His-tagged
Cat.No. : | Cstb-4002M |
Product Overview : | A DNA sequence encoding the mouse CTSB ( P10605 ) ( Met 1 - Phe 339 ) was fused with a polyhistidine tag at the C-terminus. |
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Species : | Human |
Source : | Mouse |
Tag : | His |
Protein Length : | 1-339 a.a. |
Description : | Mouse cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to the cystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. |
Form : | Lyophilized from sterile PBS , pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements. |
Purity : | > 95 % as determined by SDS-PAGE |
Bio-activity : | Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is > 1,000 pmoles / min / µg |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Stability : | Samples are stable for up to twelve months from date of receipt at -70μ |
Predicted N terminal : | His 18 |
Molecular Mass : | The secreted recombinant mouse CTSB ( pro form ) consists of 333 amino acids and has a predicted molecular mass of 36.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTSB is approximately 35-43 kDa due to glycosylation |
Storage : | Store it under sterile conditions at -70μ. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Gene Name | Cstb cystatin B [ Mus musculus ] |
Official Symbol | Cstb |
Synonyms | Cstb; Stfb; AA960480; cystatin B; cystatin-B; stefin-B |
Gene ID | 13014 |
mRNA Refseq | NM_007793 |
Protein Refseq | NP_031819 |
UniProt ID | Q62426 |
Chromosome Location | 10 C1; 10 42.0 cM |
Function | cysteine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; peptidase inhibitor activity; protease binding |
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Not For Human Consumption!
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