Active Recombinant Mouse Cst3 protein, His-tagged

• Cat.No.: Cst3-2205M
• Product Overview: Recombinant Mouse Cst3 protein(NP_034106.2)(Met1-Ala140), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 20mM HEPES, 150mM NaCl, pH 7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC. The IC50 value is < 25 nM.
• Molecular Mass: The secreted recombinant mouse CST3 consists of 131 amino acids and has a calculated molecular mass of 15 kDa. The recombinant protein migrates as three bands (15, 18 & 21-24 kDa) in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Ala140
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-140 a.a.

Gene Information

• Gene Name: Cst3 cystatin C [ Mus musculus ]
• Official Symbol: Cst3
• Synonyms: CST3; cystatin C; cystatin-C; cystatin 3; cystatin-3; CysC
• Gene ID: 13010
• mRNA Refseq: NM_009976
• Protein Refseq: NP_034106