Active Recombinant Mouse Creld2 protein, His-tagged

• Cat.No.: Creld2-492M
• Product Overview: Recombinant Mouse Creld2 protein(NP_083996.1)(Met1-Leu350), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Leu350
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of ATDC5 mouse chondrogenic cells.When 5 x 10^4 cells/well are added to mCRELD2-coated plates (0.625 μg/mL, 100 μL/well),approximately 20% cells will adhere after 60 minutes at 37°C.
• Molecular Mass: The recombinant mouse CRELD2 comprises 339 amino acids and has a predicted molecular mass of 37.5 kDa. The apparent molecular mass of the protein is approximately 51.3 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Creld2 cysteine-rich with EGF-like domains 2 [ Mus musculus ]
• Official Symbol: Creld2
• Synonyms: CRELD2; cysteine-rich with EGF-like domains 2; cysteine-rich with EGF-like domain protein 2; C85758; 5730592L21Rik
• Gene ID: 76737
• mRNA Refseq: NM_029720
• Protein Refseq: NP_083996