Active Recombinant Mouse Ces1d protein, His-tagged

• Cat.No.: Ces1d-901M
• Product Overview: Recombinant Mouse Ces1d(Tyr19-Glu561) fused with His tag at C-terminal was expressed in NS0.

Specification

• Description: Carboxylesterase 3 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. These carboxylesterases are widely distributed in mammalian tissues and have broad substrate specificity ranging from small molecule esters such as phenylester to long-chain fatty acid esters and thioesters. Because many ester-containing drugs require the ester linkage to improve the bioavailability of therapeutic agents, CESs play a major role in drug metabolism and activation. CES3 is also known as triacylglycerol hydrolase for its lipolytic activity. It has been reported to be the major lipase in white adipose tissue.
• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Tyr19
• Form: Supplied as a 0.2 μm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >11,000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 61 kDa;SDS-PAGE: 58 kDa, reducing conditions.
• Protein length: Tyr19-Glu561
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Ces1d carboxylesterase 1D [ Mus musculus ]
• Official Symbol: Ces1d
• Synonyms: TGH; Ces3; FAEE synthase; carboxylesterase 3; fatty acid ethyl ester synthase; triacylglycerol hydrolase
• Gene ID: 104158
• mRNA Refseq: NM_053200
• Protein Refseq: NP_444430
• UniProt ID: Q8VCT4
• Chromosome Location: 8; 8 C5
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Irinotecan Pathway, organism-specific biosystem
• Function: carboxylic ester hydrolase activity; fatty-acyl-ethyl-ester synthase activity; hydrolase activity