Active Recombinant Mouse Cd47 protein, Fc-tagged

• Cat.No.: Cd47-645M
• Product Overview: Recombinant Mouse Cd47(Gln19-Pro158) fused with Fc of Human IgG1 at C-terminal was expressed in NS0.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: Gln19-Pro158
• Predicted N Terminal: Gln19 predicted, no results obtained, sequencing might be blocked
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 Fc Chimera binds with an apparent KD <0.6 nM.Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is typically 2.0-8.0 μg/mL.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass: 42.3 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Publications:
  Co-coating of receptor-targeted drug nanocarriers with anti-phagocytic moieties enhances specific tissue uptake versus non-specific phagocytic clearance (2017)

Gene Information

• Gene Name: Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) [ Mus musculus ]
• Official Symbol: Cd47
• Synonyms: CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); leukocyte surface antigen CD47; Rh-related antigen; integrin-associated protein; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
• Gene ID: 16423
• mRNA Refseq: NM_010581
• Protein Refseq: NP_034711
• UniProt ID: Q61735
• Chromosome Location: 16; 16 B5
• Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hemostasis, organism-specific biosystem; Signal regulatory protein (SIRP) family interactions, organism-specific biosystem
• Function: protein binding; thrombospondin receptor activity