Active Recombinant Mouse Cd320 protein, His-tagged

• Cat.No.: Cd320-980M
• Product Overview: Recombinant Mouse Cd320 protein(NP_062294.3)(Met1-Gly208), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse CD320-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse TCN2-His, The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.
• Molecular Mass: The recombinant mouse CD320 comprises 191 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 47.7 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Gly208
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.