Active Recombinant Mouse CD274 Protein, MIgG2a mFc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD274-188MAF555
• Product Overview: The Alexa Fluor 555 conjugated extracellular domain of mouse CD274[B7-H1](NP_068693.1) (Phe19-Arg237) is fused to the N-terminus of the Fc region of mouse IgG2a was expressed in CHO cell.

Specification

• Species: Mouse
• Source: CHO
• Tag: mFc
• Protein Length: 19-237 a.a.
• Form: Lyophilized
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.
• Molecular Mass: 53 kDa
• AA Sequence: Phe19-Arg237
• Endotoxin: < 0.06 EU/ μg as determined by LAL test.
• Purity: ≥98 % by SDS-PAGE under reducing conditions.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Stable for at least 1 year after receipt when stored at -20 centigrade. Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Concentration: 100 μg/mL
• Storage Buffer: Lyophilized from 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: CD274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693