Active Recombinant Mouse Cd274 Protein, Fc-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Cd274-1707MAF647
• Product Overview: The DNA sequence encoding the extracellular domain of mouse B7-H1 (Alexa Fluor 647 conjugated) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide, inserted into a suitable mammalian expression vector and expressed in NSO cells.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Description: B7-H1, also called programmed death ligand (PD-L1) is a protein in the B7 family of immune proteins. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. Engagement of PD-L1 with PD-1 on T cells delivers a signal that inhibits TCR-mediated activation of IL-2 production and T cell proliferation. The mechanism involves inhibition of ZAP-70 phosphorylation and its association with CD247. PD-L1 signaling attenuates PKC-8 activation loop phosphorylation (resulting from TCR signaling), necessary for the activation of transcription factors NF-kappa;B and AP-1 and for the production of IL-2. Upon IFN-gamma; stimulation, PD-L1 is expressed on T cells, NK cells, macrophages, myeloid DCs, B cells, epithelial cells, and vascular endothelial cells. Type I interferons can also upregulate PD-L1 on murine hepatocytes, monocytes, DCs, and tumor cells. It appears that upregulation of PD-L1 is a mechanism that cancers can employ to evade the host immune system as patients with higher expression levels of PD-L1 had a significantly poorer prognosis.
• Form: Lyophilized
• Bio-activity: The biological activity of Mouse B7-H1 was determined by its ability to inhibit anti-CD3 induced proliferation of 72 hour PHA T cell blast. The expected ED50 for this effect is typically 2.5-10 μg/mL.
• Molecular Mass: The predicted molecular weight of Recombinant Mouse B7-H1 is Mr 51.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 75-85 kDa.
• Endotoxin: < 1.0 EU/ μg as determined by the LAL method
• Purity: > 90 % by SDS-PAGE and analyzed by silver stain
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20 to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 to 8 centigrade for one month or at -20 to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Storage Buffer: This recombinant protein was 0.2 μm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1 × PBS) pH 7.2–7.3 with no calcium, magnesium, or preservatives.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd274
• Synonyms: Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693
• UniProt ID: Q9EP73