Active Recombinant Mouse Cd101 Protein (Gln21-Phe974), C-mFc tagged

• Cat.No.: Cd101-18M
• Product Overview: Recombinant Mouse Cd101 Protein (Gln21-Phe974) with Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: NS0
• Tag: mFc
• Protein Length: Gln21-Phe974
• Description: Acts upstream of or within positive regulation of myeloid leukocyte differentiation. Predicted to be located in membrane. Predicted to be integral component of membrane. Orthologous to human CD101 (CD101 molecule).
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 1.4-8.4 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 133 kDa
  SDS-PAGE: 121-150 kDa, reducing conditions
• N-terminal Sequence Analysis: No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Arg22.
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95 %, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 400 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: Cd101 CD101 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd101
• Synonyms: Cd101; CD101 antigen; Gm734; Igsf2; Gm1016; EWI-101; immunoglobulin superfamily member 2; EWI immunoglobulin subfamily member EWI-101; glu-Trp-Ile EWI motif-containing protein 101; immunoglobulin superfamily, member 2; leukocyte surface protein
• Gene ID: 630146
• mRNA Refseq: NM_001167906
• Protein Refseq: NP_001161378
• UniProt ID: A8E0Y8