Active Recombinant Mouse ACVR2B protein(Met1-Thr134), His&hFc-tagged

• Cat.No.: ACVR2B-198M
• Product Overview: Recombinant Mouse ACVR2B (NP_031423.1)(Met 1-Thr 134) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Thr134
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind biotinylated Human INHBA-his in functional ELISA. 2. Measured by its ability to bind biotinylated mouse INHBA-his in functional ELISA. 3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell prolifeRation. The ED50 for this effect is typically 10-50 ng/mL in the presence of 10 ng/mL recombinant Activin A.
• Molecular Mass: The recombinant mouse ACVR2B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmACVR2B/Fc monomer is approximately 60-65 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Acvr2b activin receptor IIB [ Mus musculus ]
• Official Symbol: ACVR2B
• Synonyms: ACVR2B; activin receptor IIB; activin receptor type-2B; ACTR-IIB; activin receptor type IIB; ActRIIB
• Gene ID: 11481
• mRNA Refseq: NM_007397
• Protein Refseq: NP_031423