Active Recombinant Monkey BTLA Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: BTLA-195CAF555
• Product Overview: A DNA sequence encoding the Monkey BTLA (G7MKA9) (Met1-Leu155) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Monkey
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Leu155, 366
• Form: Lyophilized
• Bio-activity: Immobilized cynoBTLA-Fc at 10 μg/mL (100 μL/well) can bind biotinylated cynoTNFRSF14-Fc, EC50 of biotinylated cynoTNFRSF14-Fc is 0.7-1.64 μg/mL.
• Molecular Mass: The recombinant cynomolgus BTLA is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 41.4 kDa.The apparent molecular mass of the protein is approximately 45 kDa in SDS-PAGE.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: BTLA B and T lymphocyte associated [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated
• Gene ID: 102144877
• mRNA Refseq: XM_005548167
• Protein Refseq: XP_005548224