Active Recombinant Marmoset TNFRSF4 protein, His-tagged

• Cat.No.: TNFRSF4-340M
• Product Overview: Recombinant Marmoset TNFRSF4 protein(Leu 29 - Ala 214), fused with His tag, was expressed in HEK293.

Specification

• Species: Marmoset
• Source: HEK293
• Tag: His
• Protein Length: Leu 29 - Ala 214
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human OX40 Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Marmoset OX40, His Tag with a linear range of 0.078-1.25 μg/mL. Immobilized Marmoset OX40, His Tag at 2 μg/mL (100 μL/well) can bind Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag with a linear range of 0.2-3 ng/mL. Immobilized Marmoset OX40, His Tag at 1 μg/mL (100 μL/well) can bind Anti-OX40 Antibody, Human IgG2 with a linear range of 4-63 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 21.8 kDa. The protein migrates as 42-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.