Active Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His & Avi-tagged, Biotinylated

• Cat.No.: HA-347I
• Product Overview: Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Lys 523 (Accession # AAO46082.1). Predicted N-terminus: Asp 19. This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 60 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE). The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Influenza A H9N2
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-Influenza HA Universal MAb CM2S3 at 5 μg/mL (100 μL/well) can bind Recombinant Influenza A H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His & Avi-tagged, Biotinylated with a linear range of 1.95-31.25 ng/mL (QC tested).
• Molecular Mass: 60 kDa
• Protein length: Asp 19 - Lys 523
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.