Active Recombinant Influenza A H9N2 (A/duck/Hong Kong/448/78) HA protein(Met1-Lys523), His-tagged

• Cat.No.: HA-3235V
• Product Overview: Recombinant Influenza A H9N2 (A/duck/Hong Kong/448/78) HA protein(AAO46079.1)(Met1-Lys523), fused with His tag, was expressed in Insect Cells.

Specification

• Species: Influenza A H9N2 (A/duck/Hong Kong/448/78)
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Lys523
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.02-0.1μg/mL for 1%GRBC
• Molecular Mass: The recombinant hemagglutinin of Influenza A virus (A/duck/Hong Kong/448/78(H9N2)) consists 516 amino acids and predicts a molecular mass of 58.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.