Active Recombinant Influenza A [A/Shanghai/2/2013(H7N9)] HA protein, His-tagged

• Cat.No.: HA-1544I
• Product Overview: Recombinant Influenza A [A/Shanghai/2/2013(H7N9)] HA protein(Asp19-Gly338,Leu341-Asp523), fused with His tag, was expressed in HEK293.

Specification

• Species: Influenza A [A/Shanghai/2/2013(H7N9)]
• Source: HEK293
• Tag: His
• Protein Length: 19-338;341-523 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Influenza A [A/Shanghai/2/2013(H7N9)] HA, His Tag at 2 μg/mL (100 μL/well) can bind Influenza A H7N9 Hemagglutinin (HA) Affinity Purified Polyclonal Ab, Rabbit IgG with a linear range of 0.006-0.1 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 56.7 kDa. The protein migrates as 63-75 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Asp19-Gly338,Leu341-Asp523
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.