Active Recombinant Human Wnt-2/sFRP-1 Complex Protein

• Cat.No.: WNT2-08H
• Product Overview: Recombinant Human Wnt-2 (Ser26-Thr360, Accession # P09544.1) and Human sFRP-1 (Ser32-Lys314, Accession # AAB70793.1) Complex Protein without tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Source: CHO
• Species: Human
• Bio-activity: Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 10.0-150 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 38 kDa (Wnt-2) & 33 kDa (sFRP-1)
  SDS-PAGE: 30-45 kDa, under reducing conditions.
• N-terminal Sequence Analysis: Ser26 (Wnt-2) & Ser32 (sFRP-1)
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.

Gene Information

• Gene Name: WNT2 Wnt family member 2 [ Homo sapiens (human) ]
• Official Symbol: WNT2
• Synonyms: INT1L1; INT1L1secreted growth factor; Int-1-like protein 1; Int-1-related protein; IRP; IRPprotein Wnt-2; wingless-type MMTV integration site family member 2; Wnt2; Wnt-2
• Gene ID: 7472
• mRNA Refseq: NM_003391
• Protein Refseq: NP_003382
• MIM: 147870
• UniProt ID: P09544