Active Recombinant Human Wilms Tumor 1

• Cat.No.: WT1-1210H

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: WT-1, the product of Wilms' Tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, the inclusion or exclusion of exon 5 and three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of the WT-1 protein. Such a complex posttranscriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms' tumor. With the inclusion of exon 5, WT-1 (+KTS) binds to both DNA and RNA and is RNase but not DNase sensitive. This form also colocalizes with splicing factor in a speckled nuclear particle, suggesting that WT-1 protein may function as both a transcription factor and a splicing regulator. The WT-1 protein (residue 1-449, including exon 5 and KTS) is expressed in baculovirus system and purified by an affinity column in combination with FPLC chromatography.
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: ≥95% by SDS-PAGE.
• Activity: 1 ng is the amount sufficient for a gel mobility shift assay in a 20 μl reaction, 50 ng are sufficient for reconstituted transcription assays and 100 ng are sufficient for a protein-protein interaction assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at - 80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: WT1 Wilms tumor 1 [ Homo sapiens ]
• Synonyms: WT1; Wilms tumor 1; GUD; AWT1; WAGR; WT33; WIT-2; OTTHUMP00000037553;Wilms tumor protein
• Gene ID: 7490
• mRNA Refseq: NM_000378
• Protein Refseq: NP_000369
• MIM: 607102
• UniProt ID: Q8IYZ5
• Chromosome Location: 11p13
• Function: RNA binding; double-stranded DNA binding; metal ion binding; promoter binding; protein binding; transcription activator activity; transcription factor activity; zinc ion binding